Isolation of human testicular cells and co-culture with embryonic stem cells

Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells

  Objective(s): Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells.   Materials and Methods: A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence p...

Isolation of Adipose Tissue Stem Cells with Organ Culture Method

Background  Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...

Establishment, Culture and Freezing of Human and Mouse Embryonic Stem Cells: a Protocol Guide

Studies of the biology of human embryonic stem cells (hES cells) have developed rapidly over the past nine years since the first reports of their derivation. They clearly offer enormous potential, not only for regenerative medicine, but also for drug discovery and toxicology, human developmental biology and cancer research. Realizing these potentials a better understanding of the fundamental as...

Improvement of Expression of α6 and β1 Integrins by the Co-culture of Adult Mouse Spermatogonial Stem Cells with SIM Mouse Embryonic Fibroblast Cells (STO) and Growth Factors

Isolation, Culture, and Characterization of Human Dental Pulp Mesenchymal Stem Cells

Introduction Based on previous researches, dental pulp stem cells (DPSCs) are easily accessible with limited morbidity after collection. Their embryonic origin, from neural crests, explains their multipotency. DPSCs are primarily derived from the pulp tissues of the teeth.Objective: This study was undertaken to isolate, culture, and characterize two different third molar and first premolar hum...